Pro-Q Diamond Phosphoprotein Gel Stain

provides a method for selectively staining phosphoproteins in polyacrylamide gels. It is ideal for the identification of kinase targets in singnal transduction pathways and for phosphoproteomic sutdies. this proprietary fluorescent stain allows direct, in-gel detection of phosphate groups attached to tyrosine, serine, or threonine residues, without the need for antibodies or radioisotopes.

Pro-Q Diamond phosphoprotein gel stain allows detection of as little as 1-16 ng of phosphoprotein per band, depending on th ephosphorylation state of the protein. For individual phosphoproteinsm the strength of the singal correlates with the number of phosphate groups and is linear over three orders of magnitude. Pro-Q Diamond stain has excitation/emission maxima of 555/580 nm and can be detected using a visible-light scanning instrument, a visible-light transilluminator, or (with reduced sensitivity) a 300 nm transilluminator.

LLVY assay

Suc-LLVY-AMC是20S蛋白酶体，钙激活酶和其他类似糜蛋白酶蛋白酶的敏感荧光底物，荧光标记的底物通过AMC（Ex/Em = 351/430nm）产生亮蓝色荧光, 因此很容易的被DAPI滤光片所检测。蛋白酶体最通用的形式就是26s蛋白酶体，它包含一个20S的核心结构颗粒和两个19S调节调节基团。所有20S的核心结构颗粒包含四对七聚物环状结构组成两种不同的亚基，alpha亚基是结构亚基，beta亚基主要是催化亚基。在两个环状结构外每个含有七个alpha亚基主要是为了调整颗粒的停靠。Alpha亚基N端结构是可以阻挡未调节底物进入内腔的门户。内腔两个环分别包含七个beta亚基包含酶激活位点来执行蛋白水解反应。