Pro-Q Diamond Phosphoprotein Gel Stain

provides a method for selectively staining phosphoproteins in polyacrylamide gels. It is ideal for the identification of kinase targets in singnal transduction pathways and for phosphoproteomic sutdies. this proprietary fluorescent stain allows direct, in-gel detection of phosphate groups attached to tyrosine, serine, or threonine residues, without the need for antibodies or radioisotopes.

Pro-Q Diamond phosphoprotein gel stain allows detection of as little as 1-16 ng of phosphoprotein per band, depending on th ephosphorylation state of the protein. For individual phosphoproteinsm the strength of the singal correlates with the number of phosphate groups and is linear over three orders of magnitude. Pro-Q Diamond stain has excitation/emission maxima of 555/580 nm and can be detected using a visible-light scanning instrument, a visible-light transilluminator, or (with reduced sensitivity) a 300 nm transilluminator.